pNL(CMV)Rep78-III/CMV/WPREΔU3
(Plasmid
#190726)
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PurposeExpression of AAV2 Rep78-III in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190726 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNL4-3
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAAV2 Rep78-III
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SpeciesSynthetic
- Promoter CMV-IE
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySynthetic DNA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNL(CMV)Rep78-III/CMV/WPREΔU3 was a gift from Jakob Reiser (Addgene plasmid # 190726 ; http://n2t.net/addgene:190726 ; RRID:Addgene_190726) -
For your References section:
Efficiency and Specificity of Targeted Integration Mediated by the Adeno-Associated Virus Serotype 2 Rep 78 Protein. Li P, Marino MP, Zou J, Argaw T, Morreale MT, Iaffaldano BJ, Reiser J. Hum Gene Ther Methods. 2018 Jun;29(3):135-145. doi: 10.1089/hgtb.2018.052. 10.1089/hgtb.2018.052 PubMed 29860898