pCWU6-xylR-E-lepB
(Plasmid
#190725)
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Purposefine tune expression of type 1 signal peptidase under the control of xylose- and theophylline- inducible system
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190725 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCWU6
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namexylR-E-lepB
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GAAACAGCTATGACATGATTAC GAATTC GAGCT CTAATCTTGC ATAAACTATA TAATAAATC
- 3′ sequencing primer GTATAAATGTTAGTGATGATTATAAAA ttaTTTTACTAATCCCATTCTATTTAAAGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.04.24.538132 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCWU6-xylR-E-lepB was a gift from Chenggang Wu (Addgene plasmid # 190725 ; http://n2t.net/addgene:190725 ; RRID:Addgene_190725) -
For your References section:
Development of a Xylose-Inducible Promoter and Riboswitch Combination System for Manipulating Gene Expression in Fusobacterium nucleatum. Bibek GC, Zhou P, Naha A, Gu J, Wu C. bioRxiv. 2023 Apr 24:2023.04.24.538132. doi: 10.1101/2023.04.24.538132. Preprint. 10.1101/2023.04.24.538132 PubMed 37163003
Map uploaded by the depositor.
