CYBL-SPI1-CEBPB-IRF5
(Plasmid
#190719)
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PurposeFor microglia differentiation from iPSC
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190719 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePUCM
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSPI1-CEBPB-IRF5
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SpeciesH. sapiens (human)
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Insert Size (bp)3499
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note our NGS result found a few ambiguous bases in a repetitive region of the insert between the T2A sites. These ambiguous bases find a mixed population at these base pairs, some will affect the amino acid sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CYBL-SPI1-CEBPB-IRF5 was a gift from Li Gan (Addgene plasmid # 190719 ; http://n2t.net/addgene:190719 ; RRID:Addgene_190719) -
For your References section:
A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states. Drager NM, Sattler SM, Huang CT, Teter OM, Leng K, Hashemi SH, Hong J, Aviles G, Clelland CD, Zhan L, Udeochu JC, Kodama L, Singleton AB, Nalls MA, Ichida J, Ward ME, Faghri F, Gan L, Kampmann M. Nat Neurosci. 2022 Aug 11. pii: 10.1038/s41593-022-01131-4. doi: 10.1038/s41593-022-01131-4. 10.1038/s41593-022-01131-4 PubMed 35953545