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CYBL-SPI1-CEBPB-IRF5
(Plasmid #190719)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190719 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PUCM
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPI1-CEBPB-IRF5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3499

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note our NGS result found a few ambiguous bases in a repetitive region of the insert between the T2A sites. These ambiguous bases find a mixed population at these base pairs, some will affect the amino acid sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CYBL-SPI1-CEBPB-IRF5 was a gift from Li Gan (Addgene plasmid # 190719 ; http://n2t.net/addgene:190719 ; RRID:Addgene_190719)

  • For your References section:

    A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states. Drager NM, Sattler SM, Huang CT, Teter OM, Leng K, Hashemi SH, Hong J, Aviles G, Clelland CD, Zhan L, Udeochu JC, Kodama L, Singleton AB, Nalls MA, Ichida J, Ward ME, Faghri F, Gan L, Kampmann M. Nat Neurosci. 2022 Aug 11. pii: 10.1038/s41593-022-01131-4. doi: 10.1038/s41593-022-01131-4. 10.1038/s41593-022-01131-4 PubMed 35953545
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