G12-CASE
(Plasmid #190714)

• Purpose: Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
• Depositing Lab: Gunnar Schulte
• Publication: Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.1016/j.jbc.2022.102328.

Backbone

• Vector backbone: Clontech-style N1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: GNB3
• Species: H. sapiens (human)
• Entrez Gene: GNB3 (a.k.a. CSNB1H, HG2D)

Gene/Insert 2

• Gene/Insert name: GNGT9
• Species: H. sapiens (human)
• Tag / Fusion Protein:
  • cpVenus (N terminal on insert)

Gene/Insert 3

• Gene/Insert name: GNA12
• Species: H. sapiens (human)
• Entrez Gene: GNA12 (a.k.a. HG1M1, NNX3, RMP, gep)
• Tag / Fusion Protein:
  • Nluc is inserted at A133/F134 within GNA12

Resource Information

• Supplemental Documents:
  • pGNB3-2A-cpVenus-GNG9-IRES-GNA12-Nluc (G12-CASE).ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry