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Addgene

pVRC-N-Venus-V150A-apCC-Di
(Plasmid #190706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVRC

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    N-Venus-V150A-apCC-Di
  • Mutation
    V150A
  • Promoter araBAD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer atattgcatcagacattgcc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVRC-N-Venus-V150A-apCC-Di was a gift from Nigel Savery (Addgene plasmid # 190706 ; http://n2t.net/addgene:190706 ; RRID:Addgene_190706)

  • For your References section:

    De novo designed peptides for cellular delivery and subcellular localisation. Rhys GG, Cross JA, Dawson WM, Thompson HF, Shanmugaratnam S, Savery NJ, Dodding MP, Hocker B, Woolfson DN. Nat Chem Biol. 2022 Jul 14. pii: 10.1038/s41589-022-01076-6. doi: 10.1038/s41589-022-01076-6. 10.1038/s41589-022-01076-6 PubMed 35836017
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