pEcCas-2.0
(Plasmid
#190697)
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PurposeConstitutive expressing of Cas9 and inducible expression of lambda Red
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep15A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9 and Lamda Red system
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Alt nameCRISPR-associated protein Csn1
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SpeciesStreptococcus pyogenes
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEcCas-2.0 was a gift from Sheng Yang (Addgene plasmid # 190697 ; http://n2t.net/addgene:190697 ; RRID:Addgene_190697) -
For your References section:
Improving the Editing Efficiency of CRISPR-Cas9 by Reducing the Generation of Escapers Based on the Surviving Mechanism. Li Q, Sun M, Lv L, Zuo Y, Zhang S, Zhang Y, Yang S. ACS Synth Biol. 2023 Mar 3. doi: 10.1021/acssynbio.2c00619. 10.1021/acssynbio.2c00619 PubMed 36867054
