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Addgene

pSN879
(Plasmid #190693)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190693 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Modifications to backbone
    EGFP is replaced with the insert
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A)
  • Species
    Synthetic
  • Entrez Gene
    Kif5c (a.k.a. KINN, Khc, NKHC, NKHC2)
  • Entrez Gene
    Kif5b (a.k.a. Khc, Khcs, Kns1, Ukhc)
  • Entrez Gene
    Kif5a (a.k.a. D10Bwg0738e, Khc, Kif5, Kns, mKIAA4086)
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSN879 was a gift from Shinsuke Niwa (Addgene plasmid # 190693 ; http://n2t.net/addgene:190693 ; RRID:Addgene_190693)
  • For your References section:

    Generation of recombinant and chickenized scFv versions of an anti-kinesin monoclonal antibody H2. Niwa S, Chiba K. Cytoskeleton (Hoboken). 2023 Apr 10. doi: 10.1002/cm.21756. 10.1002/cm.21756 PubMed 37036074