pcDNA3.1-IL2sp-6His-Asprosin plasmid
(Plasmid
#190683)
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PurposeExpresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190683 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIL2-6xHis-Asprosin
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SpeciesH. sapiens (human)
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Insert Size (bp)518
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Tag
/ Fusion Protein
- IL2 signal peptide, 6xHis (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-IL2sp-6His-Asprosin plasmid was a gift from Atul Chopra (Addgene plasmid # 190683 ; http://n2t.net/addgene:190683 ; RRID:Addgene_190683) -
For your References section:
Overexpression and ELISA-based detection of asprosin in cultured cells and mice. Mishra I, Chopra AR. STAR Protoc. 2022 Dec 16;3(4):101762. doi: 10.1016/j.xpro.2022.101762. Epub 2022 Oct 13. 10.1016/j.xpro.2022.101762 PubMed 36240062