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Addgene

pReg-THP4
(Plasmid #190671)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190671 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322-ROP
  • Total vector size (bp) 5722
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    THP4
  • Species
    Synthetic
  • Insert Size (bp)
    585
  • Promoter J23103

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAAATAATTTTGTTTAAACTAGTGAACCACGAGGCCTA
  • 3′ sequencing primer AGCCTGCACTCCTCGAATTCTTAGGAT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pReg-THP4 was a gift from Andrew Ellington (Addgene plasmid # 190671 ; http://n2t.net/addgene:190671 ; RRID:Addgene_190671)

  • For your References section:

    Using fungible biosensors to evolve improved alkaloid biosyntheses. d'Oelsnitz S, Kim W, Burkholder NT, Javanmardi K, Thyer R, Zhang Y, Alper HS, Ellington AD. Nat Chem Biol. 2022 Jul 7. pii: 10.1038/s41589-022-01072-w. doi: 10.1038/s41589-022-01072-w. 10.1038/s41589-022-01072-w PubMed 35799063
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