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Addgene

HA-GFP_rescue_construct
(Plasmid #190641)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190641 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc-sgRNA-Cas9
  • Backbone manufacturer
    Ji-Long Liu
  • Backbone size w/o insert (bp) 6300
  • Total vector size (bp) 7003
  • Modifications to backbone
    double-digested with BstZ17I and SapI to disrupt sgRNA expression
  • Vector type
    Insect Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    885
  • Promoter Actin-5c
  • Tag / Fusion Protein
    • 3xHA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site FseI (not destroyed)
  • 5′ sequencing primer gagttcttgtgctgtgtgga
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-GFP_rescue_construct was a gift from David Bartel (Addgene plasmid # 190641 ; http://n2t.net/addgene:190641 ; RRID:Addgene_190641)

  • For your References section:

    Endogenous transcripts direct microRNA degradation in Drosophila, and this targeted degradation is required for proper embryonic development. Kingston ER, Blodgett LW, Bartel DP. Mol Cell. 2022 Sep 15. pii: S1097-2765(22)00849-8. doi: 10.1016/j.molcel.2022.08.029. 10.1016/j.molcel.2022.08.029 PubMed 36150386
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