pBAD-SPW
(Plasmid
#190628)
-
PurposeBacterial expression of genetically encoded protease indicator SPW
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 190628 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBAD
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSPW
-
Alt namemScarlet-I-protease substrate-mWatermelon
-
SpeciesSynthetic
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.06.20.496847 for bioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBAD-SPW was a gift from Robert Campbell (Addgene plasmid # 190628 ; http://n2t.net/addgene:190628 ; RRID:Addgene_190628) -
For your References section:
Biosensor Optimization Using a Forster Resonance Energy Transfer Pair Based on mScarlet Red Fluorescent Protein and an mScarlet-Derived Green Fluorescent Protein. Gohil K, Wu SY, Takahashi-Yamashiro K, Shen Y, Campbell RE. ACS Sens. 2023 Feb 24;8(2):587-597. doi: 10.1021/acssensors.2c01730. Epub 2023 Jan 24. 10.1021/acssensors.2c01730 PubMed 36693235