ZMYND8-dTAG
(Plasmid
#190617)
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PurposeLentiviral expression plasmid of human ZMYND8 fused with FKBP12G36V for dTAG-mediated degradation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLentiV
- Total vector size (bp) 11152
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZMYND8
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SpeciesH. sapiens (human)
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Insert Size (bp)3564
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Mutationsynonymous mutation for sgRNA resistance at aa214-218
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Entrez GeneZMYND8 (a.k.a. PRKCBP1, PRO2893, RACK7)
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Tags
/ Fusion Proteins
- FKBP12_G36V (C terminal on insert)
- Flag, 2xHA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZMYND8-dTAG was a gift from Junwei Shi (Addgene plasmid # 190617 ; http://n2t.net/addgene:190617 ; RRID:Addgene_190617) -
For your References section:
ZMYND8-regulated IRF8 transcription axis is an acute myeloid leukemia dependency. Cao Z, Budinich KA, Huang H, Ren D, Lu B, Zhang Z, Chen Q, Zhou Y, Huang YH, Alikarami F, Kingsley MC, Lenard AK, Wakabayashi A, Khandros E, Bailis W, Qi J, Carroll MP, Blobel GA, Faryabi RB, Bernt KM, Berger SL, Shi J. Mol Cell. 2021 Sep 2;81(17):3604-3622.e10. doi: 10.1016/j.molcel.2021.07.018. Epub 2021 Aug 5. 10.1016/j.molcel.2021.07.018 PubMed 34358447