pReg-FEN2
(Plasmid
#190616)
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PurposeExpresses a generalist biosensor responsive to bicyclic monoterpenes in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322-ROP
- Total vector size (bp) 5694
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFEN2
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SpeciesSynthetic
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Insert Size (bp)561
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pReg-FEN2 was a gift from Andrew Ellington (Addgene plasmid # 190616 ; http://n2t.net/addgene:190616 ; RRID:Addgene_190616) -
For your References section:
Evolving a Generalist Biosensor for Bicyclic Monoterpenes. d'Oelsnitz S, Nguyen V, Alper HS, Ellington AD. ACS Synth Biol. 2022 Jan 5. doi: 10.1021/acssynbio.1c00402. 10.1021/acssynbio.1c00402 PubMed 34985281