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Addgene

pWPT-mEGFP
(Plasmid #190606)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190606 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Plasmid 49235: pWPT-/GCCACC-mEGFP-IRES-mCherry
  • Backbone size w/o insert (bp) 10727
  • Total vector size (bp) 10729
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mEGFP
  • Species
    Synthetic
  • Insert Size (bp)
    738

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    710
  • Mutation
    a deletion was created in mCherry CDS impairing its expression
  • Promoter EIF1-short

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (destroyed during cloning)
  • 3′ cloning site PstI (destroyed during cloning)
  • 5′ sequencing primer ATAAGTGCAGTAGTCGCCGT
  • 3′ sequencing primer CATTAAAGCAGCGTATCC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWPT-mEGFP was a gift from Alessandro Quattrone (Addgene plasmid # 190606 ; http://n2t.net/addgene:190606 ; RRID:Addgene_190606)

  • For your References section:

    Translational enhancement by base editing of the Kozak sequence rescues haploinsufficiency. Ambrosini C, Destefanis E, Kheir E, Broso F, Alessandrini F, Longhi S, Battisti N, Pesce I, Dassi E, Petris G, Cereseto A, Quattrone A. Nucleic Acids Res. 2022 Oct 14;50(18):10756-10771. doi: 10.1093/nar/gkac799. 10.1093/nar/gkac799 PubMed 36165847
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