scAAV-TTR-mFgf15
(Plasmid #190594)

• Purpose: AAV construct containing Fgf15 cds under control of TTR promoter
• Depositing Lab: Roel Nusse
• Publication: Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.7554/eLife.82311.

Want a viral vector made from this plasmid?

Make a packaging request and we'll get back to you.

This service is available to academics and nonprofits only.

Please log in to submit a packaging request.

• Serotype: Select serotype for details See details about
• Pricing: Select serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
• How this works:
  • Place a request for a quantity of 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
  • Addgene will quickly confirm that we can produce a high-quality prep for you.
  • Track your request and place an order from within your account. Payment information must be added before we can begin processing your order.
  • Receive your prep in 6–9 weeks after the MTA is approved by your organization.
  • Learn more about our Packaged on Request Service.

Backbone

• Vector backbone: Derived from scAAV-TTR-CRE
• Backbone manufacturer: N/A
• Total vector size (bp): 4681
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fgf15
• Species: M. musculus (mouse)
• Insert Size (bp): 657
• Entrez Gene: Fgf15 (a.k.a. FGF19, Fgf8a)
• Promoter: Ttr

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: N/A (unknown if destroyed)
• 3′ cloning site: N/A (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

scAAV-TTR-FGF15 was produced from the complementary strand AAVS
construct, csAAV-TTR-CRE plasmid ( a kind gift of Holger Willenbring).