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scAAV-TTR-mFgf15
(Plasmid #190594)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190594 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Derived from scAAV-TTR-CRE
  • Backbone manufacturer
    N/A
  • Total vector size (bp) 4681
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fgf15
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    657
  • Entrez Gene
    Fgf15 (a.k.a. FGF19, Fgf8a)
  • Promoter Ttr

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (unknown if destroyed)
  • 3′ cloning site N/A (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

scAAV-TTR-FGF15 was produced from the complementary strand AAVS
construct, csAAV-TTR-CRE plasmid ( a kind gift of Holger Willenbring).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    scAAV-TTR-mFgf15 was a gift from Roel Nusse (Addgene plasmid # 190594 ; http://n2t.net/addgene:190594 ; RRID:Addgene_190594)

  • For your References section:

    Intermittent fasting induces rapid hepatocyte proliferation to restore the hepatostat in the mouse liver. Sarkar A, Jin Y, DeFelice BC, Logan CY, Yang Y, Anbarchian T, Wu P, Morri M, Neff NF, Nguyen H, Rulifson E, Fish M, Kaye AG, Martinez Jaimes AM, Nusse R. Elife. 2023 Jan 31;12:e82311. doi: 10.7554/eLife.82311. 10.7554/eLife.82311 PubMed 36719070
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