pNL-5.1
(Plasmid
#190589)
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PurposeLentiviral vector-mediated delivery of artificial exons for protein tagging in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190589 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNL-neo
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Vector typeLentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameNeoR transgene
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SpeciesSynthetic
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Insert Size (bp)795
- Promoter SV40
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameartificial exon encoding BSD and EGFP
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SpeciesSynthetic
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Insert Size (bp)1200
- Promoter Cellular
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNL-5.1 was a gift from Jakob Reiser (Addgene plasmid # 190589 ; http://n2t.net/addgene:190589 ; RRID:Addgene_190589) -
For your References section:
Improved tagging strategy for protein identification in mammalian cells. Bialkowska A, Zhang XY, Reiser J. BMC Genomics. 2005 Sep 4;6:113. doi: 10.1186/1471-2164-6-113. 10.1186/1471-2164-6-113 PubMed 16138932
Map uploaded by the depositor.
