p5’UTR-ORF1
(Plasmid #190566)

• Purpose: Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).
• Depositing Lab: Anthony Furano
• Publication: Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4298-303. doi: 10.1073/pnas.1416869112. Epub 2015 Mar 23.

Backbone

• Vector backbone: Derived from pUC18
• Backbone manufacturer: Multiple; e.g. Sigma-Aldrich, ThermoFisher
• Backbone size w/o insert (bp): 2720
• Total vector size (bp): 4644
• Modifications to backbone: Unknown
• Vector type: Bacterial Expression ; For DNA propagation only

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1
• Species: H. sapiens (human)
• Insert Size (bp): 1924
• Promoter: N/A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsiWI (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: M13 Forward
• 3′ sequencing primer: M13 Reverse

Resource Information

• Supplemental Documents:
  • p5'UTR-ORF1 SnapGene annotated sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is a holding vector containing contiguous regions of the human L1 (L1.3) 5’UTR and ORF1 and used to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro. Note that this plasmid was described but not named in the Supporting Information in Cook, Jones, and Furano, 2015, 10.1073/pnas.1416869112, PMID: 25831499.