pEGFP C3-Mst2
(Plasmid #19056)

• Depositing Lab: Marius Sudol
• Publication: Oka et al J Biol Chem. 2008 Jul 17. ():.

Backbone

• Vector backbone: pEGFP-C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mammalian Ste20-like kinase 2
• Alt name: serine/theronine kinase 3
• Species: H. sapiens (human)
• Insert Size (bp): 1473
• GenBank ID: NM_006281
• Entrez Gene: STK3 (a.k.a. KRS1, MST2)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mst2 cDNA insert was derived from pcDNA3.1-Flag-Mst2 construct originally obtained from Dr. Herman Sillje, Max Planck Institute of Biochemistry, Martinsried, Germany
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry