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pEGFP C3-Lats1-PY1+2 mutant
(Plasmid #19054)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19054 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Large tumor suppressor kinase 1, N mutant
  • Alt name
    Lats1, N-mutant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3393
  • Mutation
    PY1 mutant, (Tyrosine 376 changed to Alanine) and PY2 mutant (Tyrosine 559changed to Alanine)
  • GenBank ID
    NM_004690
  • Entrez Gene
    LATS1 (a.k.a. WARTS, wts)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP C3-Lats1-PY1+2 mutant was a gift from Marius Sudol (Addgene plasmid # 19054 ; http://n2t.net/addgene:19054 ; RRID:Addgene_19054)

  • For your References section:

    Mst2 and Lats kinases regulate apoptotic function of YAP. Oka T, Mazack V, Sudol M. J Biol Chem. 2008 Jul 17. ():. 10.1074/jbc.M804380200 PubMed 18640976
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