pEGFP C3-Lats1
(Plasmid #19053)

• Depositing Lab: Marius Sudol
• Publication: Oka et al J Biol Chem. 2008 Jul 17. ():.

Backbone

• Vector backbone: pEGFP-C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Large tumor suppressor kinase 1, N mutant
• Alt name: Lats1, N-mutant
• Species: H. sapiens (human)
• Insert Size (bp): 3393
• Mutation: **
• GenBank ID: NM_004690
• Entrez Gene: LATS1 (a.k.a. WARTS, wts)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

** S1111L mutation is present in Lats1. It is not known whether this has any functional consequence.