pEGFP C1-WW45
(Plasmid #19052)

• Depositing Lab: Marius Sudol
• Publication: Oka et al J Biol Chem. 2008 Jul 17. ():.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SALVADOR 1
• Alt name: SAV1, SAV, WW45
• Species: H. sapiens (human)
• Insert Size (bp): 1152
• GenBank ID: NM_021818
• Entrez Gene: SAV1 (a.k.a. SAV, WW45, WWP4)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: WW45 cDNA insert was derived from pME18SFL-WW45 construct obtained from Dr. Sumio Sugano, The Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minatoku, Tokyo, Japan.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry