Skip to main content
Addgene

mCherry-CRY2–mPKCε-CAT-HA
(Plasmid #190483)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190483 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pmCherry-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCherry-CRY2–mPKCε-CAT-HA
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
    3418
  • Entrez Gene
    Prkce (a.k.a. 5830406C15Rik, PKC[e], PKCepsilon, Pkce)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • HA (C terminal on insert)
    • Fused to CRY2 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Sma1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-CRY2–mPKCε-CAT-HA was a gift from Diomedes Logothetis (Addgene plasmid # 190483 ; http://n2t.net/addgene:190483 ; RRID:Addgene_190483)

  • For your References section:

    An optogenetic tool to recruit individual PKC isozymes to the cell surface and promote specific phosphorylation of membrane proteins. Gada KD, Kawano T, Plant LD, Logothetis DE. J Biol Chem. 2022 May;298(5):101893. doi: 10.1016/j.jbc.2022.101893. Epub 2022 Mar 31. 10.1016/j.jbc.2022.101893 PubMed 35367414
Related items: