-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 19045 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonep2xFlag CMV2
- Backbone size w/o insert (bp) 4700
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameYes-kinase associated protein
-
Alt nameYAP2, YAP65
-
Alt nameYAP2 isoform
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1467
-
GenBank IDNM_006106
-
Entrez GeneYAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
-
Tag
/ Fusion Protein
- 2xFlag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
p2xFlag CMV2-YAP2 was a gift from Marius Sudol (Addgene plasmid # 19045 ; http://n2t.net/addgene:19045 ; RRID:Addgene_19045) -
For your References section:
Mst2 and Lats kinases regulate apoptotic function of YAP. Oka T, Mazack V, Sudol M. J Biol Chem. 2008 Jul 17. ():. 10.1074/jbc.M804380200 PubMed 18640976