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Addgene

pSB1K3_M13ps_LacZα_gIII
(Plasmid #190435)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190435 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSB1K3
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    M13 packaging signal
  • Species
    Bacteriophage M13/vector M13KE

Gene/Insert 2

  • Gene/Insert name
    M13 gIII
  • Species
    Bacteriophage M13/vector M13KE

Gene/Insert 3

  • Gene/Insert name
    LacZα

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.09.02.610857 for bioRxiv preprint. Also see Pathania et al. bioRxiv 2022 (https://doi.org/10.1101/2022.05.11.491355) for related work.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB1K3_M13ps_LacZα_gIII was a gift from Manish Kushwaha (Addgene plasmid # 190435 ; http://n2t.net/addgene:190435 ; RRID:Addgene_190435)
  • For your References section:

    Phage-mediated intercellular CRISPRi for biocomputation in bacterial consortia. Pujar A, Pathania A, Hopper C, Pandi A, Függer M, Nowak T, Kushwaha M. bioRxiv 2024.09.02.610857 10.1101/2024.09.02.610857