pSB1K3_M13ps_LacZα_gIII
(Plasmid
#190435)
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PurposeM13 packaging signal, LacZα gene, and M13 gIII gene (also called gp3, or g3p) cloned into the pSB1K3 vector.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190435 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB1K3
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameM13 packaging signal
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SpeciesBacteriophage M13/vector M13KE
Gene/Insert 2
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Gene/Insert nameM13 gIII
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SpeciesBacteriophage M13/vector M13KE
Gene/Insert 3
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Gene/Insert nameLacZα
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.09.02.610857 for bioRxiv preprint. Also see Pathania et al. bioRxiv 2022 (https://doi.org/10.1101/2022.05.11.491355) for related work.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB1K3_M13ps_LacZα_gIII was a gift from Manish Kushwaha (Addgene plasmid # 190435 ; http://n2t.net/addgene:190435 ; RRID:Addgene_190435) -
For your References section:
Phage-mediated intercellular CRISPRi for biocomputation in bacterial consortia. Pujar A, Pathania A, Hopper C, Pandi A, Függer M, Nowak T, Kushwaha M. bioRxiv 2024.09.02.610857 10.1101/2024.09.02.610857