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Addgene

pSEVA63_HelperΔgIII
(Plasmid #190433)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190433 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA631
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    M13 genes I-V and VII-XI
  • Species
    Synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid contains Low Copy pBBR1 replication origin. Please visit https://doi.org/10.1101/2024.09.02.610857 for bioRxiv preprint. Also see Pathania et al. bioRxiv 2022 (https://doi.org/10.1101/2022.05.11.491355) for related work.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEVA63_HelperΔgIII was a gift from Manish Kushwaha (Addgene plasmid # 190433 ; http://n2t.net/addgene:190433 ; RRID:Addgene_190433)

  • For your References section:

    Phage-mediated intercellular CRISPRi for biocomputation in bacterial consortia. Pujar A, Pathania A, Hopper C, Pandi A, Függer M, Nowak T, Kushwaha M. bioRxiv 2024.09.02.610857 10.1101/2024.09.02.610857
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