pSEVA63_Helper
(Plasmid
#190432)
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PurposeM13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190432 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSEVA631
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameM13 genes: I-XI
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SpeciesBacteriophage M13
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains Low Copy pBBR1 replication origin. Please visit https://doi.org/10.1101/2024.09.02.610857 for bioRxiv preprint. Also see Pathania et al. bioRxiv 2022 (https://doi.org/10.1101/2022.05.11.491355) for related work.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEVA63_Helper was a gift from Manish Kushwaha (Addgene plasmid # 190432 ; http://n2t.net/addgene:190432 ; RRID:Addgene_190432) -
For your References section:
Phage-mediated intercellular CRISPRi for biocomputation in bacterial consortia. Pujar A, Pathania A, Hopper C, Pandi A, Függer M, Nowak T, Kushwaha M. bioRxiv 2024.09.02.610857 10.1101/2024.09.02.610857