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Addgene

Atg32 [200-341] 1B
(Plasmid #190431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET His6 TEV LIC cloning vector (1B)
  • Total vector size (bp) 5775
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Atg32
  • Alt name
    ECM37
  • Species
    Saccharomyces Cerevisiae
  • Insert Size (bp)
    429
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Atg32 [200-341] 1B was a gift from Michael Ragusa (Addgene plasmid # 190431 ; http://n2t.net/addgene:190431 ; RRID:Addgene_190431)

  • For your References section:

    A pseudo-receiver domain in Atg32 is required for mitophagy. Xia X, Katzenell S, Reinhart EF, Bauer KM, Pellegrini M, Ragusa MJ. Autophagy. 2018;14(9):1620-1628. doi: 10.1080/15548627.2018.1472838. Epub 2018 Aug 1. 10.1080/15548627.2018.1472838 PubMed 29909755
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