Skip to main content
Addgene

delta-BRIP1-pcDNA3.1/MycHis
(Plasmid #19042)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19042 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5493
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    delta BRIP1
  • Alt name
    BRIP1
  • Species
    H. sapiens (human)
  • Mutation
    This is a novel BRIP1 germline mutation that consists of a 4-nucleotide deletion (c.2992-2995del AAGA) in BRIP1 exon 20. It causes a shift in the reading frame and disrupts the BRCA1-binding domain.
  • GenBank ID
    AF360549
  • Entrez Gene
    BRIP1 (a.k.a. BACH1, FANCJ, OF)
  • Entrez Gene
    BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
  • Tags / Fusion Proteins
    • Myc (C terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site Apa1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    delta-BRIP1-pcDNA3.1/MycHis was a gift from Ronny Drapkin (Addgene plasmid # 19042 ; http://n2t.net/addgene:19042 ; RRID:Addgene_19042)

  • For your References section:

    A Novel Breast Cancer-Associated BRIP1 (FANCJ/BACH1) Germ-line Mutation Impairs Protein Stability and Function. De Nicolo A, Tancredi M, Lombardi G, Flemma CC, Barbuti S, Di Cristofano C, Sobhian B, Bevilacqua G, Drapkin R, Caligo MA. Clin Cancer Res. 2008 Jul 15. 14(14):4672-80. 10.1158/1078-0432.CCR-08-0087 PubMed 18628483
Related items:
Commonly requested with: