Skip to main content
Addgene

pBAD18HMGR
(Plasmid #19041)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19041 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBAD18
  • Backbone manufacturer
    Guzman et al.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase
  • Alt name
    tHMG-CoA Reductase
  • Alt name
    tHMG1
  • Alt name
    tHMGR
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    HMG1 (a.k.a. YML075C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD18HMGR was a gift from Jay Keasling (Addgene plasmid # 19041 ; http://n2t.net/addgene:19041 ; RRID:Addgene_19041)

  • For your References section:

    Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli. Pitera DJ, Paddon CJ, Newman JD, Keasling JD. Metab Eng. 2007 Mar . 9(2):193-207. 10.1016/j.ymben.2006.11.002 PubMed 17239639
Related items:
Commonly requested with: