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JAB3312
(Plasmid #190406)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190406 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    JAB2120
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone JAB2120 contains proUBQ10::PIP2A-mCherry-NOSt. Please see the fully annotated sequence linked the "Supplemental Documents" section. Please visit https://www.biorxiv.org/content/10.1101/2022.02.02.478917v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JAB3312 was a gift from Jennifer Brophy & Jose Dinneny (Addgene plasmid # 190406 ; http://n2t.net/addgene:190406 ; RRID:Addgene_190406)

  • For your References section:

    Synthetic genetic circuits as a means of reprogramming plant roots. Brophy JAN, Magallon KJ, Duan L, Zhong V, Ramachandran P, Kniazev K, Dinneny JR. Science. 2022 Aug 12;377(6607):747-751. doi: 10.1126/science.abo4326. Epub 2022 Aug 11. 10.1126/science.abo4326 PubMed 35951698
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