pABCb10aa1-70-GFP
(Plasmid
#19038)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19038 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameamino acids 1 - 70 of the targeting sequence of ABCb1
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SpeciesM. musculus (mouse)
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Insert Size (bp)70
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer EGFP-N Sequencing Primer (#6479-1): 745–724
- 3′ sequencing primer EGFP-C Sequencing Primer (#6478-1): (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pABCb10aa1-70-GFP was a gift from Orian Shirihai (Addgene plasmid # 19038 ; http://n2t.net/addgene:19038 ; RRID:Addgene_19038) -
For your References section:
Targeting, import, and dimerization of a mammalian mitochondrial ATP binding cassette (ABC) transporter, ABCB10 (ABC-me). Graf SA, Haigh SE, Corson ED, Shirihai OS. J Biol Chem. 2004 Oct 8. 279(41):42954-63. 10.1074/jbc.M405040200 PubMed 15215243