Skip to main content
Addgene

pdelta105-ABCb10-GFP
(Plasmid #19034)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19034 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ABCb10 lacking the mitochondrial targetting sequence
  • Alt name
    delta105-ABCb10
  • Alt name
    delta 105-ABCme
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2043
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer EGFP-N Sequencing Primer (#6479-1): 745–724
  • 3′ sequencing primer EGFP-C Sequencing Primer (#6478-1):
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pdelta105-ABCb10-GFP was a gift from Orian Shirihai (Addgene plasmid # 19034 ; http://n2t.net/addgene:19034 ; RRID:Addgene_19034)

  • For your References section:

    Targeting, import, and dimerization of a mammalian mitochondrial ATP binding cassette (ABC) transporter, ABCB10 (ABC-me). Graf SA, Haigh SE, Corson ED, Shirihai OS. J Biol Chem. 2004 Oct 8. 279(41):42954-63. 10.1074/jbc.M405040200 PubMed 15215243
Related items:
Commonly requested with: