LV-PE_CO-Mini
(Plasmid
#190338)
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PurposeLentiviral vector expressing PE_CO-Mini (Prime editor with codon-optimized and truncated Reverse transcriptase)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190338 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLentivector
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Vector typeLentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePE_CO-Mini, ngRNA and pegRNA for HEK3 CTTins
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.11.05.467423v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LV-PE_CO-Mini was a gift from Rasmus Bak (Addgene plasmid # 190338 ; http://n2t.net/addgene:190338 ; RRID:Addgene_190338) -
For your References section:
A truncated reverse transcriptase enhances prime editing by split AAV vectors. Gao Z, Ravendran S, Mikkelsen NS, Haldrup J, Cai H, Ding X, Paludan SR, Thomsen MK, Mikkelsen JG, Bak RO. Mol Ther. 2022 Jul 8. pii: S1525-0016(22)00422-1. doi: 10.1016/j.ymthe.2022.07.001. 10.1016/j.ymthe.2022.07.001 PubMed 35808824