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Addgene

pUC57-rm_gCarvi-T2A
(Plasmid #190334)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190334 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 2714
  • Total vector size (bp) 4748
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ratiometric gCarvi
  • Species
    Synthetic
  • Insert Size (bp)
    1947

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer M13 rev
  • 3′ sequencing primer M13 fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-rm_gCarvi-T2A was a gift from Naoto Saitoh (Addgene plasmid # 190334 ; http://n2t.net/addgene:190334 ; RRID:Addgene_190334)

  • For your References section:

    Green fluorescent cAMP indicator of high speed and specificity suitable for neuronal live-cell imaging. Kawata S, Mukai Y, Nishimura Y, Takahashi T, Saitoh N. Proc Natl Acad Sci U S A. 2022 Jul 12;119(28):e2122618119. doi: 10.1073/pnas.2122618119. Epub 2022 Jul 6. 10.1073/pnas.2122618119 PubMed 35867738
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