pKS-DTA miR-106b~25
(Plasmid
#19030)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19030 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepKS-DTA
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Vector typeMouse Targeting
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Selectable markersNeomycin (select with G418) ; none
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemicroRNA 106a-25
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Alt nameMirn25
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SpeciesM. musculus (mouse)
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Insert Size (bp)7367
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Entrez GeneMir25 (a.k.a. Mirn25, mir-25, mmu-mir-25)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKS-DTA miR-106b~25 was a gift from Tyler Jacks (Addgene plasmid # 19030 ; http://n2t.net/addgene:19030 ; RRID:Addgene_19030) -
For your References section:
Targeted deletion reveals essential and overlapping functions of the miR-17 through 92 family of miRNA clusters. Ventura A, Young AG, Winslow MM, Lintault L, Meissner A, Erkeland SJ, Newman J, Bronson RT, Crowley D, Stone JR, Jaenisch R, Sharp PA, Jacks T. Cell. 2008 Mar 7. 132(5):875-86. 10.1016/j.cell.2008.02.019 PubMed 18329372
Map uploaded by the depositor.
