pKO-II-miR-17~92
(Plasmid
#19029)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19029 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepKOII
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Vector typeMouse Targeting, Cre/Lox
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemicroRNA 17-92
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Alt nameMirn17
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SpeciesM. musculus (mouse)
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Insert Size (bp)7787
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Entrez GeneMir17 (a.k.a. Mirn17, mir-17, mmu-mir-17)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKO-II-miR-17~92 was a gift from Tyler Jacks (Addgene plasmid # 19029 ; http://n2t.net/addgene:19029 ; RRID:Addgene_19029) -
For your References section:
Targeted deletion reveals essential and overlapping functions of the miR-17 through 92 family of miRNA clusters. Ventura A, Young AG, Winslow MM, Lintault L, Meissner A, Erkeland SJ, Newman J, Bronson RT, Crowley D, Stone JR, Jaenisch R, Sharp PA, Jacks T. Cell. 2008 Mar 7. 132(5):875-86. 10.1016/j.cell.2008.02.019 PubMed 18329372