pLD-puro-MTS-EGFP
(Plasmid #190270)

• Purpose: Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells
• Depositing Lab: Christian Münch
• Publication: Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. doi: 10.21769/BioProtoc.4578. eCollection 2022 Dec 20.

Backbone

• Vector backbone: pLKO.1
• Backbone size w/o insert (bp): 7643
• Total vector size (bp): 8585
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MTS-EGFP
• Alt name: Subunit 9 of the F0-ATPase-EGFP
• Species: Synthetic
• Insert Size (bp): 942
• Promoter: Tight TRE promoter
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGCTCGTTTAGTGAACCG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: David Chan (Addgene plasmid # 23214). The open-reading frame covering Su9-EGFP was cloned into pLKO.1 background.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry