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Addgene

p21StBD
(Plasmid #190269)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190269 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    derived from pHO (51662)
  • Backbone manufacturer
    Yeast vectors for integration at the HO locus. Voth WP, Richards JD, Shaw JM, Stillman DJ. Nucleic Acids Res. 2001 Jun 15;29(12)
  • Backbone size (bp) 3100
  • Vector type
    Synthetic Biology ; Yeast genome-integrating vector
  • Promoter TDH3
  • Selectable markers
    TRP1
  • Tag / Fusion Protein
    • GAL4-BD (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p21StBD was a gift from Assaf Mosquna (Addgene plasmid # 190269 ; http://n2t.net/addgene:190269 ; RRID:Addgene_190269)

  • For your References section:

    Directed Evolution of Herbicide Biosensors in a Fluorescence-Activated Cell-Sorting-Compatible Yeast Two-Hybrid Platform. Zimran G, Feuer E, Pri-Tal O, Shpilman M, Mosquna A. ACS Synth Biol. 2022 Aug 19;11(8):2880-2888. doi: 10.1021/acssynbio.2c00297. Epub 2022 Aug 3. 10.1021/acssynbio.2c00297 PubMed 35922400
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