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Addgene

Atg11 [699-800] pHis2
(Plasmid #190264)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190264 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHis2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ATG11
  • Alt name
    CVT9
  • Alt name
    CVT3
  • Alt name
    YPR049C
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    306
  • GenBank ID
    NM_001184146
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gene was originally codon optimized and obtained as a G block.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Atg11 [699-800] pHis2 was a gift from Michael Ragusa (Addgene plasmid # 190264 ; http://n2t.net/addgene:190264 ; RRID:Addgene_190264)

  • For your References section:

    The Third Coiled Coil Domain of Atg11 Is Required for Shaping Mitophagy Initiation Sites. Margolis HK, Katzenell S, Leary KA, Ragusa MJ. J Mol Biol. 2020 Oct 2;432(21):5752-5764. doi: 10.1016/j.jmb.2020.08.025. Epub 2020 Sep 5. 10.1016/j.jmb.2020.08.025 PubMed 32896530
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