Atg11 [699-800] pHis2
(Plasmid #190264)

• Purpose: Codon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.
• Depositing Lab: Michael Ragusa
• Publication: Margolis et al J Mol Biol. 2020 Oct 2;432(21):5752-5764. doi: 10.1016/j.jmb.2020.08.025. Epub 2020 Sep 5.

Backbone

• Vector backbone: pHis2
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ATG11
• Alt name: CVT9
• Alt name: CVT3
• Alt name: YPR049C
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 306
• GenBank ID: NM_001184146
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The gene was originally codon optimized and obtained as a G block.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry