pEF-TAZ-N-Flag (S89A)
(Plasmid #19026)

• Depositing Lab: Michael Yaffe
• Publication: Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Backbone

• Vector backbone: pEF-BOS
• Backbone size w/o insert (bp): 5800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TAZ
• Alt name: WWTR1
• Alt name: Transcriptional co-Activator with PDZ-binding motif
• Alt name: WW domain containing transcription regulator 1
• Species: M. musculus (mouse)
• Insert Size (bp): 788
• Mutation: Vector has no SV40 origin, S89A (site directed mutagenesis)
• GenBank ID: NM_001168281.1 NM_133784.3
• Entrez Gene: Wwtr1 (a.k.a. 2310058J06Rik, 2610021I22Rik, Taz)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BstXI (destroyed during cloning)
• 3′ cloning site: BstXI (destroyed during cloning)
• 5′ sequencing primer: EF1a-fwd

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The murine TAZ cDNA was subcloned into pEF-BOS lacking the SV40 origin (Mizushima and Nagata, 1990) to generate pEF-TAZ. A Flag tag was inserted into the N-terminus of mTAZ cDNA by PCR, and cloned into pEF-BOS to create pEF-TAZ-N-Flag. The Murine TAZ(S89A) point mutant was constructed using the Transformer Site-Directed Mutagenesis Kit (Clontech).