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Addgene

pPOTv7-g418-spGFP(11)
(Plasmid #190257)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190257 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPOT
  • Vector type
    for endogenous gene tagging

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    spGFP(11)
  • Species
    Synthetic
  • Promoter read through

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer cTATGGGTTCTGGTAGTG
  • 3′ sequencing primer cTTAACTACCCGATCCTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.04.21.537815 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPOTv7-g418-spGFP(11) was a gift from Jack Sunter (Addgene plasmid # 190257 ; http://n2t.net/addgene:190257 ; RRID:Addgene_190257)

  • For your References section:

    Unlocking Trypanosome Biology: A Comprehensive Protein-Tagging Toolkit for Localization and Functional Analysis. Paterou A, Tyc J, Sunter J, Vaughan S, Gull K, Dean S. bioRxiv 2023.04.21.537815 10.1101/2023.04.21.537815
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