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Addgene

pEF-TAZ-N-Flag
(Plasmid #19025)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19025 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF-BOS
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TAZ
  • Alt name
    WWTR1
  • Alt name
    Transcriptional co-Activator with PDZ-binding motif
  • Alt name
    WW domain containing transcription regulator 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    788
  • Mutation
    Vector has no SV40 origin
  • GenBank ID
    NM_001168281.1 NM_133784.3
  • Entrez Gene
    Wwtr1 (a.k.a. 2310058J06Rik, 2610021I22Rik, Taz)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstXI (destroyed during cloning)
  • 3′ cloning site BstXI (destroyed during cloning)
  • 5′ sequencing primer EF1a-fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The murine TAZ cDNA was subcloned into pEF-BOS lacking the SV40 origin (Mizushima and Nagata, 1990) to generate pEF-TAZ. A Flag tag was inserted into the N-terminus of mTAZ cDNA by PCR, and cloned into pEF-BOS to create pEF-TAZ-N-Flag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF-TAZ-N-Flag was a gift from Michael Yaffe (Addgene plasmid # 19025 ; http://n2t.net/addgene:19025 ; RRID:Addgene_19025)

  • For your References section:

    TAZ: a novel transcriptional co-activator regulated by interactions with 14-3-3 and PDZ domain proteins. Kanai F, Marignani PA, Sarbassova D, Yagi R, Hall RA, Donowitz M, Hisaminato A, Fujiwara T, Ito Y, Cantley LC, Yaffe MB. EMBO J. 2000 Dec 15. 19(24):6778-91. 10.1093/emboj/19.24.6778 PubMed 11118213
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