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Addgene

pAAV-CamKII-DIO-mCherry
(Plasmid #190241)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190241 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 5593
  • Total vector size (bp) 6310
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    717
  • Promoter mouse CamKII alpha
  • Tag / Fusion Protein
    • N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Asc I (not destroyed)
  • 3′ cloning site Nhe I (not destroyed)
  • 5′ sequencing primer tcgtgtcgtgcctgagagcg
  • 3′ sequencing primer GCATTAAAGCAGCGTATCCACATAGC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CamKII-DIO-mCherry was a gift from Young J Yoon (Addgene plasmid # 190241 ; http://n2t.net/addgene:190241 ; RRID:Addgene_190241)

  • For your References section:

    Seizure-induced strengthening of a recurrent excitatory circuit in the dentate gyrus is proconvulsant. Nasrallah K, Frechou MA, Yoon YJ, Persaud S, Goncalves JT, Castillo PE. Proc Natl Acad Sci U S A. 2022 Aug 9;119(32):e2201151119. doi: 10.1073/pnas.2201151119. Epub 2022 Aug 5. 10.1073/pnas.2201151119 PubMed 35930664
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