pAAV-CamKII-DIO-hM4D(Gi)-mCherry
(Plasmid #190240)

• Purpose: Expresses iDREADD fused to mCherry flanked by DIO
• Depositing Lab: Young J Yoon
• Publication: Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119(32):e2201151119. doi: 10.1073/pnas.2201151119. Epub 2022 Aug 5.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5645
• Total vector size (bp): 7820
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hM4D(Gi)-mCherry
• Alt name: iDREADD-mCherry
• Species: H. sapiens (human)
• Insert Size (bp): 2175
• Promoter: mouse CamKII alpha
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Asc I (not destroyed)
• 3′ cloning site: Nhe I (not destroyed)
• 5′ sequencing primer: tcgtgtcgtgcctgagagcg
• 3′ sequencing primer: GCATTAAAGCAGCGTATCCACATAGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry