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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19024 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTAZ
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Alt nameWWTR1
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SpeciesM. musculus (mouse)
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Insert Size (bp)788
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Entrez GeneWwtr1 (a.k.a. 2310058J06Rik, 2610021I22Rik, Taz)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer pGEX-5 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-TAZ was a gift from Michael Yaffe (Addgene plasmid # 19024 ; http://n2t.net/addgene:19024 ; RRID:Addgene_19024) -
For your References section:
TAZ interacts with TTF-1 and regulates expression of surfactant protein-C. Park KS, Whitsett JA, Di Palma T, Hong JH, Yaffe MB, Zannini M. J Biol Chem. 2004 Apr 23. 279(17):17384-90. 10.1074/jbc.M312569200 PubMed 14970209