pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]
(Plasmid #190236)

• Purpose: AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase
• Depositing Lab: Hiroyuki Hioki
• Publication: Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi: 10.1371/journal.pone.0169611. eCollection 2017.

Backbone

• Vector backbone: pAAV-MCS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4650
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP
• Species: aequorea victoria
• Insert Size (bp): 720
• Tags / Fusion Proteins:
  • myristoylation signal sequence (N terminal on insert)
  • LDLR C-terminal sequence (C terminal on insert)
  • F2A (C terminal on insert)
  • palmitoylation signal sequence (C terminal on insert)
  • mRFP1 (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry