pAAV2-SynTetOff-palGFP
(Plasmid
#190232)
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PurposeAAV vector, high-level expression of EGFP with a palmitoylation signal derived from GAP-43 N-terminus (palGFP) in neuronal cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190232 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV-MCS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4650
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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Speciesaequorea victoria
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Insert Size (bp)720
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Tag
/ Fusion Protein
- palmitoylation signal sequence (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV2-SynTetOff-palGFP was a gift from Hiroyuki Hioki (Addgene plasmid # 190232 ; http://n2t.net/addgene:190232 ; RRID:Addgene_190232) -
For your References section:
A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. Sohn J, Takahashi M, Okamoto S, Ishida Y, Furuta T, Hioki H. PLoS One. 2017 Jan 6;12(1):e0169611. doi: 10.1371/journal.pone.0169611. eCollection 2017. 10.1371/journal.pone.0169611 PubMed 28060929
Map uploaded by the depositor.
