Skip to main content
Addgene

p40 W207R/E259A
(Plasmid #19022)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19022 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p40phox
  • Alt name
    NADPH oxidase subunit
  • Alt name
    NCF4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1019
  • Mutation
    W207R/E259A
  • GenBank ID
    X77094
  • Entrez Gene
    NCF4 (a.k.a. CGD3, NCF, P40PHOX, SH3PXD4)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p40 W207R/E259A was a gift from Michael Yaffe (Addgene plasmid # 19022 ; http://n2t.net/addgene:19022 ; RRID:Addgene_19022)

  • For your References section:

    Phosphatidylinositol 3-phosphate-dependent and -independent functions of p40phox in activation of the neutrophil NADPH oxidase. Bissonnette SA, Glazier CM, Stewart MQ, Brown GE, Ellson CD, Yaffe MB. J Biol Chem. 2008 Jan 25. 283(4):2108-19. 10.1074/jbc.M706639200 PubMed 18029359
Related items: