pMEXneo-TrkC-TrkB-hybrid
(Plasmid #190205)

• Purpose: To express a Human TrkC receptor that binds only BDNF. The second IgG like domain which is the main binding domain comes from the TrkB receptor
• Depositing Lab: Pantelis Tsoulfas
• Publication: Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40. doi: 10.1074/jbc.273.10.5829.

Backbone

• Vector backbone: pMEXneo
• Backbone manufacturer: Forough R, et al. J Biol Chem. 1993 Feb 5;268(4):2960-8. PMID: 7679105
• Backbone size w/o insert (bp): 6392
• Total vector size (bp): 8949
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TrkC
• Alt name: NTRK3 or gp145(trkC)
• Species: H. sapiens (human)
• Insert Size (bp): 2557
• Mutation: The second IgG like domain and the extracellular juxtamembrane domain are replaced with the human TrkB sequences
• GenBank ID: AAA75374.1, AAB33111.1, AAB33112.1, NP_001007157.1, NP_001012338.1, NP_001230030.1, NP_002521.2, Q16288.2,
• Entrez Gene: NTRK3 (a.k.a. GP145-TrkC, TRKC, gp145(trkC))
• Promoter: Moloney mouse sarcoma virus right LTR

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SbfI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13 Forward
• 3′ sequencing primer: EBV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry