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Addgene

pMEXneo-TrkB
(Plasmid #190203)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190203 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMEXneo
  • Backbone manufacturer
    Forough R, et al. J Biol Chem. 1993 Feb 5;268(4):2960-8. PMID: 7679105
  • Backbone size w/o insert (bp) 6366
  • Total vector size (bp) 8769
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TrkB
  • Alt name
    NTRK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2433
  • GenBank ID
    S76473 VERSION S76473.1
  • Entrez Gene
    NTRK2 (a.k.a. DEE58, EIEE58, GP145-TrkB, OBHD, TRKB, trk-B)
  • Promoter Moloney mouse sarcoma virus right LTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13-forward
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMEXneo-TrkB was a gift from Pantelis Tsoulfas (Addgene plasmid # 190203 ; http://n2t.net/addgene:190203 ; RRID:Addgene_190203)

  • For your References section:

    High resolution mapping of the binding site of TrkA for nerve growth factor and TrkC for neurotrophin-3 on the second immunoglobulin-like domain of the Trk receptors. Urfer R, Tsoulfas P, O'Connell L, Hongo JA, Zhao W, Presta LG. J Biol Chem. 1998 Mar 6;273(10):5829-40. doi: 10.1074/jbc.273.10.5829. 10.1074/jbc.273.10.5829 PubMed 9488719
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